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Contact information

_______________________

5th floor office, room B501a
Tel: +358 2941 25269

1st floor office, room B117a
Tel: +358 2941 25271

Biomedicum Helsinki
PO Box 63 (Haartmaninkatu 8)
FI-00014 University of Helsinki
Finland

Technical Information

Objectives Magnification Ojective type NA WD (mm) Ph Immersion 5x EC Plan Neofluar 0.16 18.5 Ph1 dry 10x EC Plan Neofluar 0.3 5.2 Ph1 dry 20x Plan Apochromat 0.8 0.55 Ph2 dry 40x EC Plan Neofluar 0.75 0.71   dry 40x EC Plan Neofluar 1.3 0.21   oil 63x Plan Apochromat 1.4 0.19   oil 100x EC Plan Neofluar 1.3 0.20   oil   Filters (Zeiss) Position Filter Ex(nm) Mirror(nm) Em(nm) 1 Transmitted light       2 49 Dapi 365 395 445/50 3 47HE CFP 436/25 455 480/40 4 38HE GFP 470/40 495 525/50 5 46HE YFP 500/25 515 535/30 6 31 Alexa 568 565/30 585 620/60 7 64HE mPlum 587/25 605 647/70 8 50 Cy5 640/30 660 690/50 9 25HE Dapi/Fitc/TxRed triple triple triple 10 Empty           Camera Hamamatsu Orca Flash 4.0 LT, 4 megapixel monochrome sCMOS camera Zeiss AxioCam 105 color, 5 megapixel color CMOS camera     Software Zeiss Zen 2 pro software. There are new Quick Start Guides available for ZEN Blue. They include the "First Steps", multidimensional imaging and import & export guides. Some of the functions and images refer to the ZEN Lite and are different from the full version we have, which has more functionality. There is also a video tutorial available on YouTube.

General Information

If you would like to start using the AxioImager, please go to our Fees and user information pages for instructions for new users. When you receive your user training, you will be automatically added to the Axio Imager microscope users mailing list. Please read those e-mails to know what is going on!

If you need fluorescence lamp and it is not on, first check in the paper calendar at what time it was turned off - remember that there has to be at least 30 min. gap between switching on and off the lamp.

Mosaic

The system is equiped with a motorized stage. This gives you the possibility of scanning larger image areas and combining the images into one large image.

 

Ending your imaging session

When you have finished your imaging session you should:

•  Close the electronic fluorescence light shutter

•  Log off from the computer

•  Clean objectives from oil if that was used

On working days (Mo-Fr) leave the equipment on if your reservation ends before 16:00 . If your reservation begins before 16:00 and ends after 16:00 and you are not present at 16:00 , please leave Axiovision software on to show that you will continue your imaging session.

If your session ends after 16:00 on a working day, or you are using Axioplan on Saturday, Sunday, or a holiday, you should turn off the equipment unless somebody has a reservation after you. You can check that by opening Internet Explorer. The default page of IE shows that day's reservations for Axioplan.

When turning off the equipment, the following tasks should be carried out:

•  Turn off the fluorescence light (by switching off the power supply)

• Do not disconnect the camera. The new camera gets its power from the firewire connection to the computer and will turn off when the computer is turned off.

•  Turn off the microscope and computer

•  Cover the microscope.

•  Write down the time in the paper calendar. This is important because once the mercury lamp is switched off, one needs to wait for 30 min before turning it on again.

Page updated 21.10.2016

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