Zeiss LSM 7 MP multiphoton microscope

Microscope

Motorized Zeiss Axio Examiner upright microscope

Detectors

• 2 reflected light non-descanned PMT detectors (with possibility for filters)
• 1 optional reflected light non-descanned nosepiece GaAsP detector (without filters)
• 2 transmitted light non-descanned PMT detectors, with emission filters (available on request)
• 1 transmitted light non-descanned PMT detector (available on request)

Objectives

Laser

• Coherent Chameleon Vision II, tunable wavelength: 690-1010nm.
• APE Chameleon Compact OPO (optical parametric oscillator), tunable wavelength 1050-1300nm.

Emission filters

• Reflected PMT's: BP 500-550 (green), BP 565-610 (red)
• Transmitted PMT's: BP 500-550 (green), BP 565-610 (red)

Transmitted light (available on request)

• Brightfield
• Dodt contrast (inclined illumination)

Widefield fluorescence and reflected light

• HXP 120 C metal halide lamp (fluorescence)
• LED-light source F1 (general purpose light source)

Software

• ZEN 2011 software
• LinLab software for Scientifica motorized stage

Accessories

• Dark microscope enclosure.
• Univentor gas anesthesia system with mask, induction boxes and scavenger.
• PhysioSuite heart/breath rate and oxygenation monitoring system for mice and rats.
• Animal's heating pad at the microscope stage to keep the animal warm under anesthesia.
• Scientifica adjustable motorized microscope stage with a variety of intavital animal fixation adapters.
• LED-light source F1 with flexible lightguides for manipulations under the microscope.
• Prior motorized microscope stage with multipoint scanning option (available on request).
• Dark environmental chamber (Incubator XL) for minimization of stray light (available on request).
• Heating unit for temperature control of environmental chamber (available on request).

General instructions

If you would like to start using Zeiss MP, please go to our Fees and user policy page for instructions for new users.

Important safety issue: avoid looking at the laser light - the MP laser is much more powerful than normal confocal lasers and invisible to your eyes.

Turn on the lights when you clean up, otherwise you may not notice oil spills and other problems. Cleaning up - instructions what to do after you are done with imaging.

In case of an accident - instructions what to do if you spill oil or break glass.

Always turn the main room lights off and close the microscope enclosure curtains before starting image acquisition with the microscope.

When finishing your experiment always switch the MP laser to a stand-by mode.

If the anesthesia equipment was used always disconnect the syringe with isoflurane from the machine and ventilate it for at least 10 minutes to ensure that the vaporizer is completely dry.

All registered MP users are automatically added to the MP users mailing list. Please read those e-mails to know what's going on!

Page updated 21.10.2016

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