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Biomedicum Imaging Unit

Faculty of Medicine

 

 

Biomedicum Imaging Unit (BIU)

 

 

Biocenter Finland

Euro-BioImaging

 

Contact information

_______________________

5th floor office, room B501a
Tel: +358 2941 25269

1st floor office, room B117a
Tel: +358 2941 25271

Biomedicum Helsinki
PO Box 63 (Haartmaninkatu 8)
FI-00014 University of Helsinki
Finland

Bioptonics OPT 3001 Scanner

OPT 3001

  • Location: Room 504b, 5th floor

Technical Information

Illumination System

  • White (transmission) - 8 X white LEDs
  • InfraRed (transmission) - 8 X IR LEDs
  • Fluorescence - Metal-halide 120W UV Source with liquid light guide

Filters

  • GFP: ex 425/40, em LP475
  • GFP+: ex 480/20, em LP515 (FITC, Alexa488)
  • TXR: ex 560/40, em LP610 (Cy3, Alexa594)

CCD Camera

  • Format: 1024X1024
  • Exposure range: 1 ms - 10 seconds
  • Image output: 12 bit
  • Quantum efficiency: >70%

Magnification

  • Field of View: 4X4mm ....20X20mm

Software

  • OPT scanning software
  • OPT reconstruction software:NRecon & NRecon Server
  • OPT data visualisation - DataViewer
  • OPT data analysis - CTAn
  • OPT data volume analysis - CTvol
  • OPT 3D visualisation - Bioptonics Viewer

The software can also be run on the Imaging Workstations 1 and 2

Instrument use

General Information

If you would like to start using the OPT, please go to our Fees and user information pages for instructions for new users. When you receive your user training, you will be automatically added to the OPT scanner users mailing list. Please read those e-mails to know what is going on!

If you need fluorescence illumination and it is not on, first check in the paper calendar at what time it was turned off - remember that there has to be at least 30 min. gap between switching on and off the mercury lamp.

Bioptonics OPT 3001M Scanner

Further information about the instrument can be found on the Bioptonics web pages: http://www.bioptonics.com/Home.htm

Page updated 21.10.2016

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