Nikon Eclipse Ti-E N-STORM microscope

Technical Information

Microscope

Fully motorized Eclipse Ti-E inverted microscope with Nikon IR based Perfect Focus System (PFS) and Nikon motorized XY stage.

Software

NIS-Elemnrs advanced research ver.4.2 with 6D image aquisition module, JOBS high content module, and STORM analysis.

Detectors

• Andor iXon+ 897 back-illuminated EMCCD camera (512 x 512px) (b&w)
• Andor iXon+ 885 EMCCD camera (1004 x 1002px) (b&w)

Objectives and their properties

Excitation lightsource

TIRF/STORM Lasers:

• 100mW 405nm laser
• 65mW Argon laser
• 150mW 561nm laser
• 300mW 647nm laser

Epifluorescent light:

• Nikon Intensilight metal arc lightsource

Accessories for live cell and multiwell imaging

General instructions

If you would like to start using Nikon TIRF, please go to our Fees and user policy page for instructions for new users.

Important safety issue: avoid looking at the laser light - TIRF lasers are much more powerful than the ones in laser pointers and may be harmful for your eyes.

Turn on the lights when you clean up, otherwise you may not notice oil spills and other problems. Cleaning up - instructions what to do after you are done with imaging.

In case of an accident - instructions what to do if you spill oil or break glass.

All registered TIRF users are automatically added to the TIRF users mailing list. Please read those e-mails to know what's going on!

Please contact in advance if you need temperature adjustment for your experiments.

More information on the application and principle of TIRF or STORM can be found from the MicroscopyU introduction to TIRF and STORM.

Page updated 21.10.2016

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