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Biomedicum Imaging Unit (BIU)

 

 

Biocenter Finland

Euro-BioImaging

 

Contact information

_______________________

5th floor office, room B501b
Tel: +358 9 191 25579
Fax: +358 9 191 25554

1st floor office, room B117a
Tel: +358 9 191 25271
Fax: +358 9 191 25261

Biomedicum Helsinki
PO Box 63 (Haartmaninkatu 8)
FI-00014 University of Helsinki
Finland

Nikon Eclipse Ti-E N-STORM microscope

 
  • Location: Room B115a2, 1st floor.
 
STORM News
  • The environmental chamber will need modification. In the meentime the system can only be used with a small environmetal chamber with temperature control but no CO2. You will need to use 35mm dishes (with coverglass bottoms) with this chamber. (30.9.2011)
  • The Nikon TIRF imaging system has undergone a major upgrade to a Nikon N-STORM system. This will not affect current functionality as a TIRF microscope but will add the possibility of super-resolution microscopy with the use of Stochastic Optical Reconstruction Microscopy (STORM) technology. (25.8.2011)
Technical Information

Microscope

Fully motorized Eclipse Ti-E inverted microscope with Nikon IR based Perfect Focus System (PFS) and Nikon motorized XY stage.

Software

NIS-Elemnrs advanced research ver.3.1 with 6D image aquisition module and STORM analysis.

Detectors

 

  • Andor iXon+ 897back-illuminated EMCCD camera (512 x 512px) (b&w)
  • Andor iXon+ 885 EMCCD camera (1004 x 1002px) (b&w)

Objectives and their properties

 

Magn.
Objective type
NA
Medium
Contrast
PFS
Working distance (mm)
4x
PlanFluor
0.13
Air
PhL
-
16.5
10x
PlanFluor
0.30
Air
Ph1
+
16.0
20x
PlanFluor
0.50
Air
Ph1
-
2.1
40x
PlanFluor
0.75
Air
Ph2
+
0.66
63x
 PlanApo VC
1.40
Oil
None
+
0.13
100x
 Apo TIRF
1.49
Oil
None
+
0.12
Also available
 
20x
PlanFluor
0.50
Air
DIC
2.1
40x
PlanFluor
0.75
Air
DIC
0.66
60x
PlanFluor
0.85
Air
  
0.40

Excitation lightsource

TIRF/STORM Lasers:

  • 100mW 405nm laser
  • 65mW Argon laser
  • 150mW 561nm laser
  • 300mW 647nm laser

Epifluorescent light:

  • Nikon Intensilightmetal arc lightsource

Accessories for live cell and multiwell imaging

Instrument Use

General instructions

If you would like to start using Nikon TIRF, please go to our Fees and user policy page for instructions for new users.

Important safety issue: avoid looking at the laser light - TIRF lasers are much more powerful than the ones in laser pointers and may be harmful for your eyes.

Turn on the lights when you clean up, otherwise you may not notice oil spills and other problems. Cleaning up - instructions what to do after you are done with imaging.

In case of an accident - instructions what to do if you spill oil or break glass.

All registered TIRF users are automatically added to the TIRF users mailing list. Please read those e-mails to know what's going on!

Please contact in advance if you need temperature adjustment for your experiments.

 

Links

More information on the application and principle of TIRF or STORM can be found from the MicroscopyU introduction to TIRF and STORM.

 

Page updated 03.10.2011

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